Phenotypic and Molecular Investigation of Streptococcus pneumoniae Hemolysin

The current study aimed to detect S.pneumoniae, which is one of the gram-positive bacteria that gives partial α-hemolysis on blood agar media, as well has ability complete hemolysis (beta)-hemolysis under anaerobic conditions. Fifty sputum specimens were collected from patients in Ibn sena and Al Salam hospitals Mosul/ Iraq august 2021 March 2022. Five isolates diagnosed S.pneumoniae exhibited an produce hemolysin when phenotypicallyinvestigated. Polymerase chain reaction (PCR) technique was used hlys gene Electrophoresis results showed four bands (80% isolates) with a molecular size 296 bp PCR product then sequenced.